The assignment of a String must include:

The requirement for the assignment of a String in many programming languages, particularly in those that are commonly used for robotics and STEM projects, necessitates using a specific syntax that defines the String appropriately. This includes the necessity of an identifier, which is often a variable name for the String, an equal sign to denote assignment, and the characters of the String must be enclosed in double quotes to define the start and end of the String.

Using double quotes is standard in languages like Python, Java, and C++, ensuring that the characters within are treated as a String type. The presence of the equal sign establishes the connection between the identifier (the variable name) and the value (the String itself) being assigned.

Other options incorrectly specify either additional or incorrect syntax. For instance, using single quotes, colons, or different symbols does not match the standard syntax required for defining Strings in most programming languages, which could lead to syntax errors or misinterpretation of the intended data type. Thus, the choice that includes "the word 'string', an equal sign, and double quotes around the characters" aligns perfectly with conventional programming practices for String assignment.